Diagnosis of hepatitis C virus (HCV) infection routinely performed in the microbiology laboratories is based on the detection of serum antibodies against hepatitis C virus (anti-HCV) by two main methods: enzyme immunoassay (EIA) and chemiluminescence immunoassay (CIA). Diferent reactives built on these two methods were manufactured and are still in use for the diagnosis of HCV infections. The most common problem in the laboratory screening assay of anti-HCV is the false positivity of low titers. A confrmatory test is needed to discriminate the false positive results from the accurate ones. A lot of discussions focused on deciding a reliable, easy-to-use and cost-efective confrmative test method in order to check and confrm the results of anti-HCV reactives routinely used in the detection of serum anti-HCV level.