Natural food biopreservatives have always?s remained the preference of health conscious consumers. This necessity has led to the studies exploring better alternatives which are more acceptable, economical and safer than chemical preservatives. Bacteriocins are one of such compounds produced by lactic acid bacteria which ofer a great potential to contribute in food, health and pharmaceutical industry. Present review focuses the complete biochemical, functional and molecular genetic characterization of bacteriocins produced by Pediococcus spp. A great deal of diverse heterologous expression systems have been exploited for cloning, expression and purifcation of pediocins at laboratory scale but data is lacking for industrial processes. Thus, there is an urgent need to design low cost, industrially viable and continuous system in order to exploit these natural bioactive compounds in food and pharmaceutical industry.